Miltenyi MACSQuant® Tyto®
The Chair in Cellular Immunotherapy offers users from the UKW and the University of Würzburg the opportunity to use the MACSQuant® Tyto® Cell Sorter in our facility, allowing for sterile flow-based cell sorting in a closed cartridge system.
For technical assistance & booking, contact us: hudeceklab@ukw.de
Technical Specifications
| OPTICS | |
|---|---|
| Laser excitation | 405 nm, 100 mW |
| 488 nm, 100 mW | |
| 638 nm, 100 mW | |
| Emission detectors | V1: 450/50 nm |
| V2: 525/50 nm | |
| B1: 525/50 nm | |
| B2: 585/40 nm | |
| B3: 655–730 nm | |
| B4: 750 nm LP | |
| R1: 655–730 nm | |
| R2: 750 nm LP | |
| Backscatter and side scatter for detection of size & granularity of cells | |
| Fluorescence performance | 5 decade logarithmic scales, display in lin, log, or hlog scales |
| PERFORMANCE | |
|---|---|
| Purity | >99% (dependent on input sample and Poisson statistics) |
| Cell flow rate | Up to 55,000 cells/s for the MACSQuant® Tyto® Cartridge and up to 110,000 cells/s for the MACSQuant Tyto Cartridge HS |
| Sort rate | Up to 30,000 valve actuations/s |
| Viability | >99% for lymphocytes (dependent on input sample and Poisson statistics) |
| Sorting mode | Two-way sorting of sorted and non-sorted cells |
| Technical support | Built-in live support functionality for reliable support in real time |
| Operating pressure | <230 mbar (3 psi) with the MACSQuant® Tyto® Cartridge and 1100 mbar (<14 psi) with the MACSQuant® Tyto® Cartridge HS |
| Temperature control range | 4–25 °C (under standard environmental conditions) |
| Carry-over | No sample-to-sample carry-over due to single-use disposable cartridge |
| DATA MANAGEMENT | |
|---|---|
| Measurement parameters | Height & Area measurements for all parameters |
| Signal processing | 16-bit analog-to-digital conversion and signal processing |
| Operating system | Core i7, 512 GB flash disk, 8 GB DDR4 RAM and Windows® 7 64-bit |
| Compensation | Full 8×8 matrix compensation |
| Data files | .mqd (proprietary file type); .fcs (2.0, 3.0, 3.1 compatible) |
| Data management | |
| Dimensions | 965 × 585 × 280 mm3: without external monitor |
| Weight | 62 kg (137 lbs): without external monitor |
| Monitors | 32 ̎ display and 15.6 ̎ touchscreen (internal) |
| Power requirements / consumption | 100-240 V, 50/60 Hz / 300 W: without external monitor |
| Ports | 4× USB 2.0, 6× USB 3.0, 2× Ethernet, DVI-D |
| ENVIRONMENTAL CONDITIONS | |
|---|---|
| Operational temperature / humidity | 18–25 °C / 0–85% relative humidity, non-condensating |
| Emission sound pressure < 70 dB(A) level at workstation | <70 dB(A) |
| CARTRIDGE DETAILS | |
|---|---|
| Sterility | Ethylene oxide-validated sterilization method to guarantee sterility of each cartridge |
| A-septic filters | 0.1 µm hydrophobic filters at air inlet and outlet ports |
| Sorting channel width | 25 × 50 µm |
| Maximum loading volume (input) | 100 µL–10 mL |
| Maximum loading cell number | 5×107 cells/mL |
| Cartridge alignment for laser positioning | Continuous automated cartridge alignment also during the sort |
| Cell retrieval | No cell loss - negatively sorted cells are kept in the negative collection chamber |
| Barcode labeling | Cartridges are labeled with a barcode for easy identification and traceability |
Contact us
If you are interested in joining our team, contact us via email. Please provide a Letter of Motivation stating your scientific interests, career plans and why our research is appealing to you, accompanied by your CV and two references.
Contact
Univ.-Prof. Dr. med. Michael Hudecek
Universitätsklinikum Würzburg
Department of Internal Medicine II
Oberdürrbacher Straße 6
97080 Würzburg
Germany
E-Mail: hudeceklab@ukw.de
